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| Abstract Title:
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| Living Microlens Arrays
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| Graduate Student Presenter:
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Jessica Zimberlin
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| Name of the Author(s) and Affiliation(s):
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Jessica A. Zimberlin, Polymer Science and Engineering, University of Massachusetts; Patricia Wadsworth, Biology Department, University of Massachusetts; Alfred J. Crosby, Polymer Science and Engineering, University of Massachusetts
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Using the properties of living cells and early tissue formation, we have developed a novel measurement technique to understand the mechanics related to the early stages of tissue formation of biological cells. We accomplish this by defining adaptable surface structures of three-dimensional, hexagonal arrays of microlenses. These “living” microlenses are achieved by growing a monolayer cell sheet on a thin film of polystyrene [PS] attached to a substrate of crosslinked poly(dimethyl siloxane) [PDMS] microwells. The contractile nature of the cells attached to the surface and the compliance of the PDMS surface geometry allows the PS thin film to buckle, forming arrays of convex microlenses. The curvature of the microlens structures is related to the strain applied by the monolayer cell sheet attached to the PS surface. We use this measurement to differentiate the strains applied by two different cell types and relate these strains to differences in the intercellular coupling of the different cell types. We also show that by adding different chemical triggers to the system, the contractile nature of the cells changes, modifying the focal length of the microlenses. These microlenses have been demonstrated to focus light and images, and therefore truly represent a "living" technology. This design introduces a new paradigm for advanced materials and offers great promise for a range of applications.
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