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| Abstract Title:
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| Barcoding of Silica particles with Multicolored Semiconducting Quantum Dots
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| Graduate Student Presenter:
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Gerson Aguirre
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| Name of the Author(s) and Affiliation(s):
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A. Couzis; C. Maldarelli; L. Gilchrist; CCNY Chemistry and CCNY Chem. Eng. Dept.
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The focus of this project is to synthesize silica particles that can encapsulate luminescent Cd/Se-Zn/S core-shell hydrophobic molecule-capped nanocrystals (QDs). These particles should range from 1 μm-20 μm depending on the delivery system whereby the QDs will allow for the construction of the barcode as a variation of the number of colors and the number of QDs pertaining to each color are controlled, i.e. multiplexing. The particles are synthesized in a surfactant-free emulsion system of base-catalyzed polymerization of Dimethyldiethoxysilane as a precursor and secondly the addition of tetraethylorthosilicate. The hybrid organic/inorganic cross-linked particles allows for the encapsulation of QDs into a vestibule in which a metaloxide shell can be grown and still maintain the biologically important properties. Lipobeads will be made and then arrayed onto the surface of a micron size wells etched on a patterned silicon grid and with the use of the luminescent QDs will create the barcode. The array would have approximately hundreds of thousand registries on a chip with each having the capability of displaying a different receptor. Binding events of the membrane receptor in the bilayer will be detected using fluorescence microscopy. The advantages of this barcoding method over others is that the delivery system does not damage the proteins via shearing or denaturing as in fluidic spotting techniques, and due to the volume of proteins required to immobilize the proteins on the surface of the particles, leads to dramatic miniaturization.
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